In fluorescence microscopy, the sample of what is studied is the light source. Fluorescence microscopy is centered around the idea that various materials emit visible light when irradiated with the light from a specific wavelength. Some samples are naturally fluorescent such as chlorophyll.
The image is created when the microscope has a filter that sorts our the strength of the light emitted and matches the wavelength with the material. When the radiation collides with the atom in the specimen, electrons are excited to a higher energy level. The light is the separated with another filter, and are projected in the microscope with high contrast against a dark background.
Fluorescence microscopy is rapidly expanding in the medical and biological field due to its abilities to identify cellular composition with great detail and accuracy.
