Wednesday, September 4, 2013

Fluorescence Microscopy Tips

The images you take are only as good as your equipment. So take time to take care of your equipment on a regular basis; clean objectives, cover slips, and ensure your microscope has the proper setup.

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Most objectives are designed for a cover-slip that is .17mm thick. If you fail to use the proper cover-slip, you will lose image clarity and sharpness. Although cover-slip thickness can range anywhere from .08mm to .25mm in thickness, .17mm is the most common and optimal for most microscopes.

Infinite EPI-Fluorescent Trinocular Compound Microscope 40-1000x

Slides are also another component that vary in quality. Research your brands and find out which is the best for your type of microscope.

One Pack of 100 Piece Blank Glass Slides with Frosted End

For further tips we recommend referencing the following text:
"A Guide to Super-Resolution Fluorescence Microscopy" (2010) Schermelleh et al. J Cell Bio.
"Live Cell Microscopy- Tips and Tools" (2009) Frigault et al. J Cell Sci.


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