Wednesday, May 1, 2013

What is Phase Contrast Microscopy?

Phase contrast is a microscopy method used by Fritz Zernike. Zernike made multiple discoveries including the direct path of light. Phase contrast microscopy makes images appear darker against a light background.  Phase contrast uses a condenser system and an objective lens. Phase is only useful on images that are colorless or transparent because it makes it difficult to see it's surroundings. Objects suitable for phase contrast microscopy are bacteria, protozoa, and cells. Zernike was awarded the Nobel Peace Prize in 1953 for his discoveries in Physics.

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